Establishment of experimental animal model of osteoporosis induced by ovariectomy in rats

Objective To investigate the establishment of osteoporosis model induced by ovariectomy in rats.Methods Thirty old female Wistar rats were randomly divided into two groups:the ovariectomized group and sham ovariectomized group.The bone density of right lateral thigh was observed with X-ray and the serum ALP activity was measured pre-and post operation using an ALP-kit.Histological changes of the right lateral thigh were observed by HE stain.Results After three months,the bone density of ovariectomized group was decreased more than that of the SHAM group,and the serum ALP activity of ovariectomized group was significantly higher than that of the SHAM group(P0.05) .Histological observation of ovariectomized rats showed pathological changes of osteoporosis,displaying bone trabecularism porosity and medullary canal enlargement.Conclusions The animal model of osteoporosis induced by ovariectomy in rats was feasible and lateral thigh bone of OP model was fit for study of implantation.