Isolation and Physiology Characteristics of Sulfate-reducing Bacteria

Sulfate-reducing bacteria (SRB) was isolated from the sediment collected from oxidation pond of Yaer Lake, a seriously polluted lake by direct discharge of chloralkali effluent. After incubation in the laboratory, the physiology characteristics of SRB and its effect of environment factors such as temperature, pH, salinity, ferrous ion and sulfide were studied. Results indicated that this strain was Gram-negative, which only grew with sodium lactate as carbon source and sulfate as sulfur source under anaerobic condition, and hydrogen sulfide was generated in this process. The optimum conditions for SRB growth were found to be at 35℃, pH 7.0, salinity 0.7%, ferrous ion 0.5g/L and without the existence of sulfide.