The binding and degradation of adaptor proteins TAB2 by Trim6 inhibit TAB2-induced NF-κB activation

To investigate the protein interaction between Trim6 and TAB2,moreover to identify the effects of Trim6 on NF-κB luciferase reporter gene activation induced by TAB2,the interaction between Trim6 and TAB2 was examined by coimmunoprecipitation.Then,whether Trim6 could target TAB2 for degradation was detected by Western blot.Furthermore,the effects of Trim6 on the NF-κB activation induced by TAB2 were determined by dual-luciferase reporter assay.Coimmunoprecipitation experiments indicated that Trim6 interacted with TAB2.Besides,Trim6 could target TAB2 for degradation.As a result,dual-luciferase assay showed that Trim6 could effectively block TAB2-induced NF-κB activation.The result of this study suggests that Trim6 was involved in negative regulation of TAB2-induced NF-κB activation though interacting with,and degrading TAB2.