Establishment of radioresistant subline from human nasopharyngeal carcinoma cell line by repeating irradiation

Objective To establish radioresistant cell subline from a human nasopharyngeal carcinoma cell line CNE-3 and investigate the mechanism of radioresistance.Methods Radioresistant cell subline were established from a human nasopharyngeal carcinoma cell line CNE-3 by repeating X-ray irradiation as radiotherapy.The radiosensitivity of this subline,together with its parent CNE-3 cell line,were measured.Results This subline,named CNE-3R could be subcultured steadily three months,and kept SF20.5.Conclusion Radioresistant cell subline from a human nasopharyngeal carcinoma cell line successfully was established by using the method of research,which providing the tools for the study of the mechanism of radioresistance.