Relative mRNA Expression Levels of Frizzed Family Genes in Mice Ovaries at Different Estrous Stages

Real-time Q-PCR was used to analyze the relative expression levels of Frizzed 1-10 mRNAs in the mice ovaries at different estrous stages.The results showed that they had different expression patterns except Frizzed 8.Expressions of Frizzed 1 and 2 were the highest in proestrus and both were about 4 times higher than those in diestrus(P0.05).Frizzed 3 was 1.5 fold higher in proestrus and diestrus than those in the other two periods(P0.05).Frizzed 4 was found significantly higher expression in estrus,which is about 3 fold than that in diestrus(P0.05).Frizzed 5 was marked higher in diestrus than those in proestrus and estrus,being approximately 5 times higher than that in estrus(P0.05).Frizzed 7 was similar to Frizzed 5,about 4 fold higher than the level in proestrus(P0.05).Frizzed 10 expression in proestrus was about more than 3 times higher than that in estrus(P0.05),but Frizzed 6 and 9 were no significant difference during the estrous cycle(P0.05).These results suggested that Frizzeds were widely and differentially expressed in estrous ovary and Frizzed 1—4 may play a more important role in the development of follicle during the estrous cycle.