Hair growth-promoting effects of Sargassum glaucescens oligosaccharides extracts

• Microwave-assisted high-pressure water enhanced S. glaucescens fucoidan yield • Subcritical water extraction can directly generate oligo-fucoidans. • Fucoidan extracts promoted human follicle dermal papilla cells proliferation. • Fucoidan extracts enhanced IGF-1 and LEF-1 gene expression and promote hair growth. • Low molecular weight fucoidan has hair growth-promoting activity in C57BL/6 mice. Background : Brown algae have been used as food and traditional Chinese medicine for thousands of years and it is known to contain a bioactive component - fucoidan. However, little is known about the effect of fucoidan on cell differentiation and proliferation, particularly in topical application. Given that, this study focused on investigating the effect of different extraction methods of fucoidans on the promotion of human hair growth ability. Methods : Fucoidans from Sargassum glaucescens were extracted by a combination of microwave-assisted high-pressure water or subcritical water. Different molecular weight fractions of fucoidan were separated. The effect of these fractions on the proliferation and hair growth-related genes expression on hair follicle dermal papilla cells (HFDPC) was investigated. Mice models and ex vivo hair follicle culture assays were used to determine the efficacy. Significant Findings: Sargassum glaucescens fucoidan extracts higher than 1 KDa molecular weight demonstrated hair growth-promoting ability. The oligo-fucoidan was more effective in enhancing hair follicle cell proliferation. The effects of fucoidans on the proliferation of HFPDC correlated with IGF-1 and LEF-1 gene expressions. Hair growth-promoting activity was observed in mice models after being treated with Sargassum glaucescens fucoidan extracts. Thus, fucoidan could be a potential topical medicine in alopecia treatment. :