Induksi Kalus Tanaman Kentang (Solanum tuberosum L.) varietas Granola dari Jenis Eksplan yang Berbeda dengan Zat Pengatur Tumbuh 2,4-DSecara in Vitro

This experiment was knew effect of: a) Plant Growth Regulator (ZPT) 2,4-Dichlorofenoxyacetic acid, b) Different explant, c) interaction of ZPT 2,4-D and different explant on the callus induction of potato plant varietas Granola. This experiment was performed on November 2013 – March 2014 in laboratory Tissue Culture YAHDI zone of Perum Pelabuhan in Jl. Lambung No. 16 Medan Marelan. Design of experiment is Completelly Randomized Design (RAL) factorial. First factor is ZPT 2,4-D with 4 stage treatment (0, 1, 2, 3 ppm) and second factor is different explant include root, stem and leaf. Total Combination of treatment is 12 with 4 repetition. Observation did 28 day. Parameter observed were time of calus formed, callus teksture, callus colour, callus biomass, callus wide surface. Result of experiment: there is effect very significant a) ZPT 2,4-D, b) different explant, c) interaction of ZPT 2,4-D and different explant on the callus of biomass and wide surface. Explant of stem potensial to form callus on 1 and 2 ppm 2,4-D treatment on 14 day induction. Explant of root potensial to formed callus 3 ppm 2,4-D treatment on 15 day induction and 2 ppm 2,4-D treatment on 16 day induction. Explant of leaf isn’t form callus. Callus colour from explant of root with 2 and 3 ppm treatment is result white-chocelate colour. Explant of stem with 1 ppm 2,4-D is result white-yellow colour, 2 and 3 ppm 2,4-D treatment is result white-green colour. All of callus having friable tekstur. Treatment of 3 ppm ZPT 2,4-D and explant of stem result higher of callus biomass is1,14 gram with wide surface is 1,60 cm2.