检出限
适体
结直肠癌
生物传感器
胶体金
化学
组合化学
寡核苷酸
介电谱
电极
色谱法
材料科学
纳米技术
纳米颗粒
DNA
电化学
癌症
生物化学
分子生物学
医学
生物
内科学
物理化学
作者
Mihaela Tertis,Petrica Ionut Leva,Diana Bogdan,Maria Suciu,Florin Graur,Cecilia Cristea
标识
DOI:10.1016/j.bios.2019.05.012
摘要
Despite the fact that cancer research has experienced important advances and remarkable improvement in the curing processes during the last decades, this disease still occupies a leading position among the causes of death worldwide. It has been demonstrated that there is an interconnection between the overexpression of interleukin-6 cytokine and the tumor growth, metastasis, and therapeutic resistance in several types of malignancies. Herein, a highly sensitive and selective aptasensor for quantitative detection of interleukin-6 was developed by using a glassy carbon electrode modified with p-aminobenzoic acid, p-aminothiophenol and gold nanoparticles. A thio-terminated aptamer specific for interleukin-6 was immobilized on the surface of the modified electrode via the formation of gold-sulfur bonds. This DNA oligonucleotide was then used as a detection probe to capture the target protein at the biosensor surface allowing label-free detection by electrochemical impedance spectroscopy. The developed aptasensor showed a good linear response from 5 pgmL-1 to 100 ngmL-1 with a detection limit of 1.6 pgmL-1, within the range of physiological concentration of the protein. The biosensor exhibited high selectivity and has been successfully used to detect interleukin-6 in blood samples collected from patients suffering of colorectal cancer, with excellent recoveries after the addition of known amount of the target protein.
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