纤维化
再生(生物学)
成纤维细胞
肌腱
细胞生物学
细胞外基质
基因剔除小鼠
病态的
功能(生物学)
细胞
生物
肌成纤维细胞
癌症研究
病理
囊性纤维化
核糖核酸
医学
成纤维细胞生长因子
损失函数
细胞生长
肺纤维化
免疫学
基质(化学分析)
作者
Ruifu Lin,Junchao Luo,Hong Zhang,Chunmei Fan,Yue Hu,Ruojin Yan,Zetao Wang,Yang Fei,Chenqi Tang,Tianxi Huang,Tianshun Fang,Weiliang Shen,Sunbin Ling,Hongwei Ouyang,Xiao Chen,Zi Yin
标识
DOI:10.1038/s41467-025-67355-7
摘要
Tissue fibrosis is a major cause of organ dysfunction. Preventing fibrosis in tissue pathological condition remains a significant clinical challenge. Here we investigate the role of fibroblast growth factor 7 (FGF7) in mitigating fibrosis and promoting regeneration of load-bearing tendons. Fgf7 knockout mice exhibit impaired motor function and disordered matrix assembly in tendons. Single-cell RNA sequencing reveals an enrichment of a pro-fibrotic cell subpopulation in Fgf7-deficient tendons, which is also predominant in human tendinopathy. Using ProTracer technology, we find that FGF7 deficiency drives proliferating cells toward this pro-fibrotic lineage. Furthermore, we find that FGF7 promotes the tenogenic differentiation of tendon stem/progenitor cells while suppressing their fibrotic differentiation. Importantly, a hydrogel loaded with recombinant FGF7 effectively reduces fibrosis and significantly promotes functional tendon regeneration in vivo. These findings elucidate FGF7's dual role in driving tenogenesis and inhibiting fibrosis, suggesting a potential therapeutic strategy.
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