去细胞化
自愈水凝胶
类有机物
组织工程
细胞外基质
纳米技术
再生医学
材料科学
多细胞生物
生物医学工程
计算机科学
细胞生物学
干细胞
生物
工程类
细胞
高分子化学
遗传学
作者
Beatriz S. Moura,Maria V. Monteiro,Luís P. Ferreira,Pedro Lavrador,Vítor M. Gaspar,João F. Mano
标识
DOI:10.1002/adfm.202202825
摘要
Abstract The extracellular matrix plays a critical role in bioinstructing cellular self‐assembly and spatial (re)configuration processes that culminate in human organoids in vitro generation and maturation. Considering the importance of the supporting matrix, herein is showcased the most recent advances in the bioengineering of decellularized tissue hydrogels for generating organoids and assembloids. Key design blueprints, characterization methodologies, and extracellular matrix processing toolboxes are comprehensively discussed in light of current advances. Such enabling approaches provide the grounds for engineering next‐generation tissue‐specific hydrogels with close‐to‐native biomolecular signatures and user‐tailored biophysical properties that may potentiate organoids physiomimetic potential. In a forward looking perspective, the combination of tissue‐specific decellularized hydrogels with increasingly complex multicellular assemblies and bottom‐up cell engineering technologies may unravel unprecedented tissue‐like physiological responses and further advance the exploitation of organoids and assembloids as human disease surrogates or as patient‐tailored living therapeutics.
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