Re-Du-Ning injection ameliorates radiation-induced pneumonitis and fibrosis by inhibiting AIM2 inflammasome and epithelial-mesenchymal transition.

医学 蛋白激酶B 上睑下垂 炎症体 药理学 PI3K/AKT/mTOR通路 癌症研究 信号转导 免疫学 炎症 生物 细胞生物学
作者
Chenxi Yang,Chenglin Song,Yi Wang,Wencheng Zhou,Wei Zheng,Han Zhou,Guoliang Deng,Haibo Li,Wei Xiao,Zhong-Qi Yang,Ling‐Dong Kong,Hui Ming Ge,Yaohong Song,Yang Sun
出处
期刊:Phytomedicine [Elsevier BV]
卷期号:102: 154184-154184 被引量:26
标识
DOI:10.1016/j.phymed.2022.154184
摘要

Radiation-induced lung injury (RILI) is a common side effect in chest radiotherapy patients, and there is no good medicine to treat it. Re-Du-Ning (RDN) injection is a traditional Chinese medicine that is clinically used to treat upper respiratory tract infections and acute bronchitis. RDN has the advantage of high safety and mild side effects. The mechanism of most traditional Chinese medicine preparations is unknown.To illustrate the mechanisms of RDN for the treatment of RILI.Female C57BL/6 mice were used to establish a RILI model via irradiation, and RDN injection was intraperitoneally administered at doses of 5, 10, and 20 ml/kg. The cytokines were measured by ELISA and qPCR. The data related to Absent in melanoma 2 (AIM2) inflammasome were analyzed via ELISA and a network pharmacological approach. In addition, the data related to epithelial-mesenchymal transition (EMT) were analyzed via immunofluorescence, Western blotting, and a network pharmacological approach.RDN robustly alleviated RILI. Meanwhile, RDN downregulated inflammatory cells' infiltration and the expression of pro-inflammatory cytokines, such as IL-1β, IL-6, and TNF-α. Next, the potential molecular mechanisms of RDN were predicted through network pharmacology analysis. RDN may ameliorate radiation pneumonitis (RP) by inhibiting AIM2-mediated pyroptosis. Moreover, RDN treatment inhibited EMT and phosphoinositide-3-kinase (PI3K)/protein kinase B (AKT) pathway. The active compounds from Lonicera japonica Thunb. decreased the phosphorylation of Akt.These findings demonstrate that RDN, as a traditional Chinese medicine preparation, will be a candidate drug for treating RILI.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
zlzlzl发布了新的文献求助10
刚刚
852应助czb采纳,获得10
刚刚
地质学一点完成签到,获得积分10
刚刚
秀丽的大门完成签到,获得积分20
1秒前
了该发布了新的文献求助10
1秒前
乐观小之应助www采纳,获得10
4秒前
怕黑的凡灵完成签到 ,获得积分10
7秒前
周四一发布了新的文献求助60
8秒前
ddaikk完成签到,获得积分20
10秒前
10秒前
朴实成风完成签到 ,获得积分10
10秒前
13秒前
13秒前
亚亚完成签到,获得积分20
14秒前
NexusExplorer应助骤雨时晴采纳,获得10
16秒前
hanch发布了新的文献求助20
17秒前
DRZZZ发布了新的文献求助10
17秒前
忧郁雪糕完成签到 ,获得积分10
18秒前
酷炫的煎饼完成签到,获得积分20
18秒前
18秒前
小蘑菇应助ddaikk采纳,获得10
18秒前
亚亚发布了新的文献求助10
18秒前
雨后阳光完成签到,获得积分10
19秒前
19秒前
吕苗苗发布了新的文献求助20
20秒前
善学以致用应助Whale采纳,获得10
20秒前
21秒前
21秒前
21秒前
科研通AI2S应助修脚大师采纳,获得10
22秒前
谢书繁发布了新的文献求助10
22秒前
哈哈完成签到,获得积分10
22秒前
23秒前
23秒前
甜美梦槐发布了新的文献求助10
24秒前
温元冬完成签到,获得积分10
24秒前
zyw完成签到,获得积分10
24秒前
坚强的雁蓉完成签到 ,获得积分10
24秒前
25秒前
26秒前
高分求助中
Thinking Small and Large 500
Algorithmic Mathematics in Machine Learning 500
Mapping the Stars: Celebrity, Metonymy, and the Networked Politics of Identity 400
Getting Published in SSCI Journals: 200+ Questions and Answers for Absolute Beginners 300
The direct observation of dislocations 200
Reference Guide for Dynamic Models of HVAC Equipment 200
A Treatise on Hydrostatics and Hydrodynamics 200
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 物理 生物化学 纳米技术 计算机科学 化学工程 内科学 复合材料 物理化学 电极 遗传学 量子力学 基因 冶金 催化作用
热门帖子
关注 科研通微信公众号,转发送积分 3836569
求助须知:如何正确求助?哪些是违规求助? 3378884
关于积分的说明 10506466
捐赠科研通 3098617
什么是DOI,文献DOI怎么找? 1706576
邀请新用户注册赠送积分活动 821075
科研通“疑难数据库(出版商)”最低求助积分说明 772431