适体
石墨烯
检出限
胶体金
纳米技术
材料科学
聚醚酰亚胺
电位传感器
生物传感器
纳米颗粒
化学
电位滴定法
色谱法
电极
聚合物
复合材料
生物
遗传学
物理化学
作者
Guiyin LI,Bó Wáng,Le Zhao,Xiaohang Shi,Guanxiong Wu,Wei Chen,Lixian SUN,Jianwei Liang,Zhide Zhou
标识
DOI:10.1016/j.electacta.2022.140808
摘要
Glypican-3 (GPC3) protein is considered as an effective and specific biomarker for the accurate diagnosis of hepatocellular carcinoma (HCC), and there is a critical demand to construct strategies with shorter time, lower cost noise and higher sensitivity. In this study, a biosensor for label-free detection of GPC3 was prepared by modifying GPC3 aptamer (GPC3Apt) on the surface of Si-based light addressable potentiometric sensor (LAPS) chip modified by reduced graphene oxide/polyetherimide/gold nanoparticles (rGO/PEI/AuNPs). Among them, GPC3Apt with a low equilibrium dissociation constant was used as a biorecognition molecule to recognize and capture GPC3. The rGO/PEI/AuNPs decorated on the LAPS surface could enlarge the surface area for GPC3Apt immobilization and provide more active sites for GPC3Apt to bind effectively with the target GPC3. When a bias voltage was provided to the LAPS system, the forming GPC3-GPC3Apt complexs on the sensing interface of LAPS would cause the potential of the sensitive membrane surface to change and generate a corresponding offset response in photocurrent-voltage (I-V) curves. The potential shift and GPC3 concentration showed a good linear range from 0.1 to 100.0 μg/mL with a detection limit of 40.0 ng/mL (S/N = 3) and a sensitivity of 1.33638 mV/μg/mL. In addition, the LAPS detection system also has good specificity and stability, which can be used for the detection of GPC3 in spiked serum samples.
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