萨奎纳维尔
端粒酶
外周血单个核细胞
分子生物学
生物
DNA合成
刀豆蛋白A
细胞生长
体外
化学
免疫学
生物化学
病毒
基因
病毒载量
抗逆转录病毒疗法
作者
Ornella Franzese,Angela Lombardi,Alessandro Comandini,Elda Cannavò,C Testorelli,Ilaria Cirello,Enzo Bonmassar
出处
期刊:Life Sciences
[Elsevier]
日期:2001-08-01
卷期号:69 (13): 1509-1520
被引量:8
标识
DOI:10.1016/s0024-3205(01)01243-7
摘要
The present study describes the effect of Saquinavir on proliferation, interferon-gamma production and telomerase activity of non-stimulated, or activated non-adherent mononuclear cells (NAMNC), obtained from peripheral blood of healthy donors. Fresh NAMNC, non-stimulated or activated in vitro with PHA or with a mixture of monoclonal antibodies against CD3 and against CD28 membrane antigens (in order to obtain prevalent T cell responses), were exposed to Saquinavir before or at the time of mitogenic stimulation. Control and treated cells were tested for DNA synthesis (3H-thymidine incorporation), interferon-gamma production and telomerase activity (TRAP assay). The results indicate that Saquinavir is able to increase proliferation and interferon-gamma release in PHA-stimulated NAMNC, and telomerase activity either in non-stimulated and in PHA or antibody-activated cells. These results suggest that the activity against HIV infection afforded by Saquinavir, could be corroborated by its effects on the host. These include its adjuvant activity on mitogen-induced responses of lymphocytes, and its possible antagonistic effects against lymphoid cell senescence, through telomerase activation.
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