In Vitro and In Vivo Evaluation of Chlorogenic Acid-Encapsulated Lignin on Patulin Adsorption and Alleviation of Patulin-Induced Colonic Damage

Patulin (PAT) is a water-soluble mycotoxin that causes digestive tract damage and liver and kidney function abnormalities. The current control approaches only reduce the amount of PAT in raw materials and food, which is difficult to remove once ingested in the body. In this study, lignin-based cross-linked particles loaded with chlorogenic acid were prepared, which intervened the liver and colon damage caused by PAT in mice. In the simulated digestion process in vitro, the accumulated adsorption capacity of the LB/CA-SH for PAT was 0.934 mg/g. LB/CA-SH intervention reversed the shortening of the colon length, alleviated the changes in the activities of antioxidant enzymes, and reduced the levels of oxidation markers protein carbonyl and malondialdehyde in the colon tissue of the model group. The absorption of sorbent alleviated the decrease of organ index and the abnormality of serum biochemical indexes (alanine aminotransferase, aspartate aminotransferase, urea nitrogen, and uric acid) caused by PAT. These results support the potential of using LB/CA-SH as a novel protective agent to reduce the toxicity of PAT.