The study examined the impact of Professional Development (PD) on preschool teachers' content knowledge (CK), pedagogical content knowledge (PCK), and scaffolding practice. In a pre-post-follow-up design, 77 teachers were assigned to three groups (EG1: block play curriculum materials + PD, EG2: block-play curriculum materials, CG: no materials). Results showed improvements in teachers' scaffolding after the PD, but no changes in CK or PCK. The use of block-play curriculum materials and scaffolding was associated with an improvement in children's math knowledge, but not with their stability knowledge. The study highlights the need for practice-oriented PD aligned with preschool teachers' everyday practice. • Scaffolding-focused PD improved preschool teachers' instructional strategies. • Reflective practice was effectively encouraged through brief demonstrations. • PD improved children's math knowledge but not their stability theories. • Short-term PD can enhance teaching practices when aligned with daily routines.