The mRNA Vaccine Expressing Single and Fused Structural Proteins of Porcine Reproductive and Respiratory Syndrome Induces Strong Cellular and Humoral Immune Responses in BalB/C Mice

免疫系统 生物 抗原 抗原性 病毒学 免疫学 接种疫苗 信使核糖核酸 脾脏 抗体 病毒 免疫荧光 基因 遗传学
作者
Luoyi Zhou,Ashenafi Kiros Wubshet,Jiangrong Zhang,Shitong Hou,Kaishen Yao,Qiuyi Zhao,Junfei Dai,Yongsheng Liu,Yaozhong Ding,Jie Zhang,Yuefeng Sun
出处
期刊:Viruses [Multidisciplinary Digital Publishing Institute]
卷期号:16 (4): 544-544 被引量:7
标识
DOI:10.3390/v16040544
摘要

PRRS is a viral disease that profoundly impacts the global swine industry, causing significant economic losses. The development of a novel and effective vaccine is crucial to halt the rapid transmission of this virus. There have been several vaccination attempts against PRRSV using both traditional and alternative vaccine design development approaches. Unfortunately, there is no currently available vaccine that can completely control this disease. Thus, our study aimed to develop an mRNA vaccine using the antigens expressed by single or fused PRRSV structural proteins. In this study, the nucleotide sequence of the immunogenic mRNA was determined by considering the antigenicity of structural proteins and the stability of spatial structure. Purified GP5 protein served as the detection antigen in the immunological evaluation. Furthermore, cellular mRNA expression was detected by immunofluorescence and western blotting. In a mice experiment, the Ab titer in serum and the activation of spleen lymphocytes triggered by the antigen were detected by ELISA and ICS, respectively. Our findings demonstrated that both mRNA vaccines can significantly stimulate cellular and humoral immune responses. More specifically, the GP5-mRNA exhibited an immunological response that was similar to that of the commercially available vaccine when administered in high doses. To conclude, our vaccine may show promising results against the wild-type virus in a natural host.
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