Dual-Nanozyme Cascade for System-Wide Specific Colorimetric Detection of Aminoglycoside Antibiotics

acts as the second substrate for colorimetric detection. Using gentamicin (GMC) as a proof of concept, we established a detection range of 0.1-10 μg/mL, with a detection limit (LOD) of 91 ng/mL. In addition, we validated the colorimetric response for other AGs and employed principal component analysis (PCA) for differentiating among various AGs. This approach enables nanozymes to selectively recognize their targets through a cascade mechanism. Furthermore, it facilitates the simultaneous detection and identification of antibiotics, highlighting its potential for in-field applications.