病毒学
干扰素
生物
冠状病毒
病毒
先天免疫系统
下调和上调
突变体
病毒复制
猪流行性腹泻病毒
微生物学
免疫系统
免疫学
2019年冠状病毒病(COVID-19)
医学
基因
遗传学
疾病
病理
传染病(医学专业)
作者
Kexin Yan,Simeng Feng,Jiahao Cheng,Fan Zheng,Zhiqiang Li,Zhibang Deng,Xiying Yuan
标识
DOI:10.1016/j.vetmic.2022.109622
摘要
Interferon-induced transmembrane proteins (IFITMs) play an important role in the innate immune response triggered by viral infection. Transmissible gastroenteritis virus (TGEV) causes severe diarrhea, vomiting and dehydration in piglets, resulting in huge economic losses to the swine industry. In this study, we showed that IFITM3 inhibits the replication of TGEV and interferes with the binding of TGEV to PK15 cells. Moreover, the inhibitory effect of IFITM3 on TGEV circumvents the upregulation of inflammatory cytokines. Subsequently, we found that the M22A mutant loses part of the antiviral effect of IFITM3 on TGEV; in contrast, the K24A mutant enhances the antiviral effect of IFITM3. Notably, our data shows a synergistic effect between IFITM3 and CQ, which further amplifies the antiviral effect against TGEV.
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