The hepatoprotective effect of traditional liquid medicine containing curcuma, turmeric, and red ginger on rat liver damage with paracetamol-induced hepatotoxicity

Many factors, one of which is drug toxicity, can cause liver damage. The metabolism of the analgesic/antipyretic paracetamol in the liver can result in liver toxicity. Curcumin and gingerol, two active compounds found in natural products, have been shown to be antioxidants. The objective of this study was to examine how curcuma, turmeric, and red ginger in traditional liquid medicine (TLM) affected the antioxidant and hepatoprotective effects of paracetamol-induced liver damage in rats. In rats treated with paracetamol with or without TLM, levels of the enzymes alanine aminotransferase (ALT), aspartate aminotransferase (AST) and malondialdehyde (MDA) were assayed. Silymarin (a milk thistle derivative with possible hepatoprotective effects) was used as a positive (treated) control. In comparison to the negative control group (paracetamol alone), the TLM was able to prevent a significant rise in MDA. The best prevention was with a TLM dose of 10.08 mL/KgBW, with an 87.80 percent prevention of increased plasma MDA levels. Compared to the negative group, this dose also showed the highest hepatoprotective activity as reflected by AST and ALT levels decreasing by 35.4 percent and 51.2 percent, respectively. In addition, the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay was used to evaluate the antioxidant activity of traditional medicinal products. TLC-Densitometry was utilized to analyze curcumin marker compounds, whereas High Performance Liquid Chromatography (HPLC) was used to analyze gingerol. Using the DPPH method, the IC50 value of TLM was 74.0658 ppm. TLM's marker compound contained 1.325 percent curcumin and 0.20 percent gingerol. by decreasing MDA, AST and ALT levels, it can be concluded that TLM, which contains curcuma, turmeric, and red ginger, has potential antioxidant and hepatoprotective activity in rats induced by paracetamol.