Breaking‐down Tumoral Physical Barrier by Remotely Unwrapping Metal‐Polyphenol‐Packaged Hyaluronidase for Optimizing Photothermal/Photodynamic Therapy‐Induced Immune Response

Abstract The therapy of solid tumors is often hindered by the compact and rigid tumoral extracellular matrix (TECM). Precise reduction of TECM by hyaluronidase (HAase) in combination with nanotechnology is promising for solid tumor therapeutics, yet remains an enormous challenge. Inspired by the treatment of iron poisoning, here we propose a remotely unwrapping strategy of metal‐polyphenol‐packaged HAase (named PPFH) by sequentially injecting PPFH and a clinically used iron‐chelator deferoxamine (DFO). The in situ dynamic disassembly of PPFH could be triggered by the intravenously injected DFO, resulting in the release, reactivation and deep penetration of encapsulated HAase inside tumors. Such a cost‐effective HAase delivery strategy memorably improved the subsequent photothermal and photodynamic therapy (PTT/PDT)‐induced intratumoral infiltration of cytotoxic T lymphocyte cells and the cross‐talk between tumor and tumor‐draining lymph nodes (TDLN), thereby decreasing the immunosuppression and optimizing tumoricidal immune response that can efficiently protect mice from tumor growth, metastasis and recurrence in multiple mouse cancer models. Overall, this work presents a proof‐of‐concept of the dynamic disassembly of metal‐polyphenol nanoparticles for extracellular drug delivery as well as the modulation of TECM and immunosuppressive tumor microenvironment. This article is protected by copyright. All rights reserved