丙酮酸
谷氨酰胺分解
瓦博格效应
厌氧糖酵解
生物
代谢途径
分解代谢
细胞生物学
糖酵解
生物化学
新陈代谢
作者
Guanning Su,Jiao Liu,Chenrui Duan,Puxian Fang,Liurong Fang,Yanrong Zhou,Shaobo Xiao
出处
期刊:Redox biology
[Elsevier]
日期:2024-05-01
卷期号:71: 103112-103112
标识
DOI:10.1016/j.redox.2024.103112
摘要
The Warburg effect, also referred as aerobic glycolysis, is a common metabolic program during viral infection. Through targeted metabolomics combined with biochemical experiments and various cell models, we investigated the central carbon metabolism (CCM) profiles of cells infected with porcine deltacoronavirus (PDCoV), an emerging enteropathogenic coronavirus with zoonotic potential. We found that PDCoV infection required glycolysis but decreased glycolytic flux, exhibiting a non-Warburg effect characterized by pyruvic acid accumulation. Mechanistically, PDCoV enhanced pyruvate kinase activity to promote pyruvic acid anabolism, a process that generates pyruvic acid with concomitant ATP production. PDCoV also hijacked pyruvic acid catabolism to increase biosynthesis of non-essential amino acids (NEAAs), suggesting that pyruvic acid is an essential hub for PDCoV to scavenge host energy and metabolites. Furthermore, PDCoV facilitated glutaminolysis to promote the synthesis of NEAA and pyrimidines for optimal proliferation. Our work supports a novel CCM model after viral infection and provides potential anti-PDCoV drug targets.
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