This chapter provides a comprehensive exploration of bio-based polyamides as environmentally sustainable alternatives to traditional counterparts. Beginning with a brief overview of polyamides and their widespread applications in industries such as automotive, textiles, and packaging, the narrative highlights the environmental concerns associated with conventional polyamide production, leading to the critical need for sustainable alternatives. The chapter focuses on the importance of bio-based polyamides, derived from sources such as castor oil, agricultural feedstocks, and microbial synthesis. It delves into production methods, comparing chemical synthesis and biotechnological approaches, and evaluates their properties, including mechanical strength, thermal stability, chemical resistance, and biodegradability. The exploration of sources, production techniques, and applications positions bio-based polyamides as key players in the transition toward a more environmentally conscious future in polymer science. The chapter concludes with a forward-looking perspective, emphasizing the transformative role bio-based polyamides can play in achieving a harmonious balance between industrial functionality and ecological responsibility.