Mercury(编程语言)
荧光
水溶液
化学
色谱法
物理
计算机科学
有机化学
量子力学
程序设计语言
作者
Xiao Zhu,Xiaoming Zhou,Da Xing
标识
DOI:10.1016/j.bios.2010.03.013
摘要
A new label-free Hg2+ ions assay with polymerase assisted fluorescence amplification was developed. In this approach, a single strand template probe containing a mercury ion recognition sequence (MRS) which is composed of two thymine (T)-rich functional areas separated by a spacer of random bases was designed. In this sensing system, upon addition of Hg2+ ions, the complexation of Hg2+ ions with the MRS yielded a hairpin complex through T–Hg2+–T base pairs. The formation of the hairpin structure initiated the replication of the template probe in the presence of the polymerase/dNTPs. Then the replication products of long duplex DNA probes were stained with a sensitive reagent SYBR Green I (SG). As a result, SG integrated with the duplex DNA probes and induced a distinguishable fluorescence enhancement in response to Hg2+ ions triggered replication reaction. Due to the fluorescence amplification step, this method exerted substantial enhancement in sensitivity and could be used for rapid and selective detection of low picomolar Hg2+ ions. It is expected that this cost-effective fluorescence sensor might hold considerable potential in the detection of Hg2+ ions in real environmental samples.
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