蛋白核小球藻
脂质代谢
生物合成
基因
脂质积聚
生物化学
酶
生物
营养水平
磷酸烯醇式丙酮酸羧化酶
淀粉
基因表达
化学
小球藻
植物
藻类
生态学
作者
Jianhua Fan,Yanbin Cui,Yang Zhou,Minxi Wan,Weiliang Wang,Junxia Xie,Yuanguang Li
标识
DOI:10.1016/j.biortech.2014.04.087
摘要
Heterotrophy to photoautotrophy transition leads to the accumulation of lipids in Chlorella, which has potential to produce both healthy food and biofuels. Therefore, it is of key interest to study the metabolism shift and gene expression changes that influenced by the transition. Both total and neutral lipids contents were increased rapidly within 48 h after the switch to light environment, from 24.5% and 18.0% to 35.3% and 27.4%, respectively, along with the sharp decline of starch from 42.3% to 10.4% during 24 h photoinduction phase. By analyzing the correlation between lipid content and gene expression, results revealed several genes viz. me g3137, me g6562, pepc g6833, dgat g3280 and dgat g7566, which encode corresponding enzymes in the de novo lipid biosynthesis pathway, are highly related to lipid accumulation and might be exploited as target genes for genetic modification. These results represented the feasibility of lipid production through trophic converting cultivation.
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