Ultrasensitive fluorescence detection of transcription factors based on kisscomplex formation and the T7 RNA polymerase amplification method

Herein, we report a kisscomplex based protein fluorescence assay (KPFA) method, which employed the formation of a kisscomplex and the T7 RNA polymerase amplification method, for the assay of transcription factors with high sensitivity. The detection limits of MITF and NF-κB p65 are 0.23 pM and 0.496 pM, respectively.