Detection of subgroup J avian leukosis virus infection in Australian meat‐type chickens

Objective To determine the extent of avian leukosis virus subgroup J (ALV‐J) infection in Australian broiler breeder flocks, using virus isolation and molecular biological detection. Any resultant ALV‐J viral isolates to be characterised by neutralisation cross testing in order to determine antigenic relationships to overseas isolates of ALV‐J. Study design Samples of blood, feather pulp, albumen and tumours were obtained from broiler breeder flocks which represented four genetic strains of meat chickens being grown in Victoria, South Australia, NSW and Queensland. Dead and ailing birds were necropsied on farm and samples were collected for microscopic and virological examinations. Virus isolation was carried out in C/O and DF‐1 CEF cultures and ALV group specific antigen was detected in culture lysates using AC‐ELISA. Micro‐neutralisation assay was used for antigenic characterisation of selected isolates. Genomic DNA was isolated from cultured cells, tumours and feather pulp. ALV‐J envelope sequences were amplified by PCR using specific ALV‐J primers while antibodies against ALV‐J were detected by ELISA. Results A total of 62 ALV‐J isolates were recovered and confirmed by PCR from 15 (31.3%) of 48 breeder flocks tested. Antibody to ALV‐J was detected in 20 (47.6%) of the 42 flocks tested. Characteristic lesions of myeloid leukosis caused by ALV‐J were found in affected flocks. The gross pathological lesions were characterised by skeletal myelocytomas located on the inner sternum and ribs, neoplastic enlargement of the liver, and in some cases gross tumour involvement of the spleen, kidney, trachea, skeletal muscles, bone marrow, skin and gonads. Microscopically, the tumours consisted of immature granulated myelocytes, and were present as focal or diffuse infiltrations in the affected organs. Virus micro‐neutralisation assays demonstrated antigenic variation among Australian isolates and to overseas strains of ALV‐J. Conclusion ALV‐J infection was prevalent in Australian broiler breeder flocks during 2001 to 2003. Australian isolates of ALV‐J show a degree of antigenic variation when compared to overseas isolates.