脱氧核酶
分析物
分析灵敏度
核酸
检出限
化学
细菌
微生物培养
大肠杆菌
条形码
细菌生长
DNA
纳米技术
计算生物学
色谱法
计算机科学
生物
生物化学
材料科学
基因
操作系统
病理
医学
替代医学
遗传学
作者
Richa Pandey,Dingran Chang,Marek Śmieja,Todd Hoare,Yingfu Li,Leyla Soleymani
出处
期刊:Nature Chemistry
[Springer Nature]
日期:2021-06-24
卷期号:13 (9): 895-901
被引量:62
标识
DOI:10.1038/s41557-021-00718-x
摘要
The detection and identification of bacteria currently rely on enrichment steps such as bacterial culture and nucleic acid amplification to increase the concentration of target analytes. These steps increase assay time, cost and complexity, making it difficult to realize a truly rapid point-of-care test. Here we report the development of an electrical assay that uses electroactive RNA-cleaving DNAzymes (e-RCDs) to identify specific bacterial targets and subsequently release a DNA barcode for transducing a signal onto an electrical chip. Integrating e-RCDs into a two-channel electrical chip with nanostructured electrodes provides the analytical sensitivity and specificity needed for clinical analysis. The e-RCD assay is capable of detecting 10 CFU (equivalent to 1,000 CFU ml–1) of Escherichia coli selectively from a panel containing multiple non-specific bacterial species. Clinical evaluation of this assay using 41 patient urine samples demonstrated a diagnostic sensitivity of 100% and specificity of 78% at an analysis time of less than one hour compared with the several hours needed for currently used culture-based methods. Methods to detect and identify bacteria typically rely on enrichment steps such as bacterial culture and nucleic acid amplification. Now, an assay for detecting bacteria based on a two-channel electrical chip that combines electroactive DNAzymes with an electrochemical readout, has been developed. This assay enables reagentless and culture-free detection of bacteria in clinical samples.
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