卵泡闭锁
颗粒细胞
细胞凋亡
细胞生物学
下调和上调
窦卵泡
生物
卵泡发生
卵泡期
程序性细胞死亡
卵巢
内分泌学
卵泡
内科学
医学
生物化学
低温保存
基因
胚胎
作者
Carolina Marvaldi,Daniel Martin,Julia G Conte,María Florencia Gottardo,Matías L. Pidre,Mercedes Imsen,Martín Irizarri,Sharrόn L. Manuel,Francesca E. Duncan,Vı́ctor Romanowski,Adriana Seilicovich,Gabriela Jaita
出处
期刊:Reproduction
[Bioscientifica]
日期:2021-05-01
卷期号:161 (5): 581-591
被引量:6
摘要
Humanin (HN) is a short peptide involved in many biological processes such as apoptosis, cell survival, inflammatory response, and reaction to stressors like oxidative stress, between others. In the ovary, a correct balance between pro- and anti-apoptotic factors is crucial for folliculogenesis. In the follicular atresia, survival or death of granulosa cells is a critical process. The goal of this study was to evaluate the action of HN on granulosa cell fate. To explore endogenous HN function in the ovary, we used a recombinant baculovirus (BV) encoding a short-hairpin RNA targeted to silence HN (shHN). HN downregulation modified ovarian histoarchitecture and increased apoptosis of granulosa cells. HN was also detected in a granulosa tumor cell line (KGN). Transduction of KGN cells with BV-shHN resulted in HN downregulation and increased apoptosis. On the other hand, treatment of KGN cells with exogenous HN increased cell viability and decreased apoptosis. In summary, these findings indicate that HN is a cytoprotective factor in granulosa cells of antral follicles, suggesting that this peptide would be involved in the regulation of folliculogenesis. Also, this peptide is a cytoprotective factor in KGN cells, and therefore, it could be involved in granulosa tumor cell behavior.
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