Molecular characterization of a porcine kobuvirus strain isolated in Fujian,China

The objective is to understand the complete genome and the genome organization of the porcine kobuvirus isolated in Fujian(PKV-FJ),China.Reverse transcription polymerase chain reaction were used to amplified the target gene fragments of the PKV-FJ,and the rapid-amplification of cDNA ends methods were employed to obtained the terminals for the virus using the 5′/3′RACE Kit(2nd generation).The full-genome of the PKV-FJ was 8 210bp including a single open reading frame(ORF)of 7 467bp encoding apolyprotein with 2 488amino acid.The 5′untranslated regions was 576bp long,while the3′untranslated regions was 167bp long.The polyprotein nucleotide sequences shared the highest identities with CH/HNXX-4/2012at 89.2%,and the lowest with swine/S-1-HUN/2007/Hungary at 87.6%,respectively.