Effect of Selenium Chitosan on Expression of bcr /abl Fusion Gene in Human Chronic Myelogenous Leukemia Cell Line K562

Objective: To investigate the effects of selenium chitosan(Sc) on proliferation of human chronic myelogenous leukemia cell line K562 and explore its relationship with the expression of bcr /abl fusion gene.Method: After treatment with Sc,cell proliferation was determined with SRB and clonal assay.The abundance of P210bcr/ablprotein was examined with Western blotting.The expression of bcr /abl fusion gene was detected by RT-PCR.Result: Exposure of K562 cells to 50,100 and 200 mg·L-1 of Sc,respectively,for 48h could obviously inhibit proliferation and decrease their survival rate(P 0.05,P 0.01);After treatment with 50,100,200 mg·L-1 of Sc,respectively,for 48 h or 200 mg.L-1 of Sc for 12,24,and 48 h,the abundance of P210bcr/ablprotein in K562 cells was down-regulated in a concentration and time-dependent manner(P 0.05,P 0.01),but the expression of bcr /abl fusion gene was not changed.Conclusions: Sc could inhibit the proliferation of K562 cells cultured in vitro,and this effect was correlated with down-regulation of the abundance of P210bcr/abl proteins encoded by bcr /abl fusion gene.