Prokaryotic expression of human nervous system polycomb 1(NSPc1) and preparation of anti-NSPc1 polyclonal antibody

Objective To express the human recombinant NSPc1 protein and prepare specific polyclonal antibody against it.Methods The recombinant expression plasmid pET43.1a-HIS-NSPc1 was constructed and then transformed into E.coli.(BL21),the recombinant fusion protein HIS-NSPc1 was expressed and purified.Four New Zealand rabbits were immunized with purified recombinant HIS-NSPc1 protein and polyclonal antibody against NSPc1 was prepared.The specificity of the anti-sera was analyzed by Western Blot.Results The purity of the recombinant NSPc1 protein is up to about 95%.Rabbit against NSPc1 antibody was obtained.Western blot result showed that the antibody was able to detect both endogenous and/or exogenous NSPc1.Conclusion The NSPc1 polyclonal antibodies prepared by using recombinant NSPc1 protein as antigen has high specificity to NSPc1.It can be used for functional analysis of NSPc1 in vivo and in vitro.