Effect of 14 kD protein activator from Botrytis cinerea on induced resistance of tomato gray mould disease and correlative enzyme activity

A 14 kD protein elicitor from Botrytis cinerea,which can induce tomato to gain resistance of gray mould disease,was researched by the method of pot culture in the green house.The induc- ing efficiency reached 67.03 % at 17th day post inoculation of pathogen when seeds were dipped in 1.182μg/ml activator protein.To reveal the mechanism of resistance induction,the dynamic varia- tion of PAL,POD,PPO which relate with resistance metabolism were measured.The activity of Phenylalanine ammonia lyase(PAL)Peroxides(POD)and polypheol oxidase(PPO)were in- creased effectively after inducement The activity of PAL increased by 53.82 % at 48h.The maxi- mum activity of POD was 2.63 times the control group and occurred at 168 h.The activity of PPO increased by 77.57 % and 87.21% at 24 h and 120 h,respectively.The enhancement of activity of the enzyme correlated with defense is one of the main physiological mechanisms of protein activator inducing disease resistance.