Technological study on cellulose degradation with cellulase by cross experiments

The technological conditions of degrading cellulose of haulm by cross experiments were studied with cellulase.The experimental results showed by cross experiments that the influential extent of affecting factors to degrading cellulose of haulm is:A(Enzyme amount)B(Substrate concentration)E(Reaction time)C(Reaction temperature)D(pH).The best combination of degrading cellulose of haulm with cellulase is A3B1E3C3D2,that is,the optimal conditions of degrading cellulose are 0.2% of cellulase,5.0% of haulm,at 50℃ and pH 5.0 for 2.0hrs.The ratio of cellulose to glucose reached 22.3% within only two hours which the time is shortened 12-30 times compared with the traditional enzymatic way.