Effects of Grape Procyanidins on the Proliferative Activity and the Conc entration of Intracellular Calcium of Hepatoma Cells

BACKGROUNDAIM: To investigate the effects of grape procyanidins on the proliferative activity and the concentration of intracellular calcium of hepatoma cells. MATERIAL AND METHODS: Human hepatoma cells were cultured with differrent concentrations of GPC.The proliferative activity and concentration of intracellular calcium of the hepatoma cells were measured by methyl thiazolyl tetrazolium(MTT)assay and microfluorimetric techniques based on calcium indicator Fura_2/AM. RESULTS: The proliferation level and concentration of intracellular calcium of the control group were(0.045±0.024)nmol/L and(130.53±17.48)nmol/L,respectively.The cell proliferation activitives of3GPC groups were lower,but the concentration of intracellular calcium was higher with increasing amounts of GPC(t2.78,P0.05).The concentration of intracellular calcium of both50mg/L and100mg/L GPC group were overloaded. CONCLUSION: GPC could inhibit the proliferative activity and abnormally elevate the concentration of intracellular calcium of hepatoma cells.