Identification of the Downstream Targets of Nanog by Chromatin Immunoprecipitation

Objective:To identify the downstream targets of Nanog by chromatin immunoprecipitation.Methods:By formaldehyde cross-linking and sonicationg,the chromatin of embryonic stem cells was splitted to fragments with an aver-age length approximately 0.5～1 kb.The DNA fragments binding to targeted protein Nanog were riched by immunoprecipi-tation,and were isolatede and purificated after reverse crosslinks.The combination of Nanog and DNA fragments was proved by electrophoretic mobility shift assay(EMSA).Results:Our data shows that the following screened gene is fzr.We proved the results by EMSA.Conclusion:Fzr is important for the regulation of cell cycle.This elucidates the mechanism of Nanog function elementarily.