The application of USB2.0 communication technology in ultraviolet-visible spectrophotometer is introduced.The speed of data transfers between ultraviolet-visible(UV-VIS) spectrophotometer and PC is higher,compared with RS232 bus.The practical application shows that the system adopting USB2.0 bus possesses the feature of high speed and real time.It reduces time of data transmission between spectrophotometer and PC,thus enhancing the performance of the whole instrument.