基因沉默
核酸
小干扰RNA
感应(电子)
核糖核酸
化学
DNA
RNA干扰
RNA沉默
核糖核酸酶P
基因
分子生物学
细胞生物学
生物
生物化学
物理化学
作者
Jianbing Li,Xuehe Lu,Tiantian Wu,Xiaohui Wu,Lin Han,Baoquan Ding
标识
DOI:10.1002/anie.202011174
摘要
Abstract Chemically modified DNA has been widely developed to fabricate various nucleic acid nanostructures for biomedical applications. Herein, we report a facile strategy for construction of branched antisense DNA and small interfering RNA (siRNA) co‐assembled nanoplatform for combined gene silencing in vitro and in vivo. In our design, the branched antisense can efficiently capture siRNA with 3′ overhangs through DNA–RNA hybridization. After being equipped with an active targeting group and an endosomal escape peptide by host–guest interaction, the tailored nucleic acid nanostructure functions efficiently as both delivery carrier and therapeutic cargo, which is released by endogenous RNase H digestion. The multifunctional nucleic acid nanosystem elicits an efficient inhibition of tumor growth based on the combined gene silencing of the tumor‐associated gene polo‐like kinase 1 (PLK1). This biocompatible nucleic acid nanoplatform presents a new strategy for the development of gene therapy.
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