TRPA1-mediated inflammatory response of alveolar epithelial cells in vitro experiment

Introduction. TRPA1 channels have been repeatedly studied in experimental models of inflammation. There are evidences in the modern literature confirming the expression of TRPA1 receptors on the alveolar epithelial cells. One of the known natural agonists of these channels is cinnamaldehyde (CA). Aim . To evaluate the effect of TRPA1 receptors on the development of an inflammatory response in A549 alveolar epithelial cells, as an in vitro model of inflammation in the respiratory tract. Materials and methods. The supernatant of A549 human adenocarcinoma cell culture was collected after exposure to various doses of CA and the levels of 13 cytokines were determined in the samples. Results. It has been found that A549 cells produce such cytokines as MCP1, IL6, IL8 and IL33. It was established that, when exposed to CA at low concentrations, the production of MCP-1, IL6 and IL8 increased, but high concentrations of CA conversely decreased production of the cytokines. Under preliminary exposition with TRPA1 selective antagonist (HC-030031) subsequent stimulation with 100 μM CA did not elicit increase in cytokines concentration. Conclusion . Thus, we demonstrated the peculiarities of TRPA1-mediated inflammatory response in in vitro model of A549 alveolar epithelium.