毛囊
窦卵泡
卵母细胞
男科
体外
卵泡
生物
卵泡发生
体外受精
胚胎
胚胎发生
内分泌学
内科学
胶原酶
卵泡期
细胞生物学
医学
生物化学
酶
作者
Li Wei Ang,Cao Hai-Xia,Li HongXia,Li Ruijiao,Guo Xingping,Huaixiu Wang
出处
期刊:Zygote
[Cambridge University Press]
日期:2020-11-25
被引量:2
标识
DOI:10.1017/s096719942000060x
摘要
Summary The present study investigated the effects of c-type natriuretic peptide (CNP) on the development of murine preantral follicles during in vitro growth (IVG). Preantral follicles isolated from ovaries of Kunming mice were cultured in vitro . In the culture system, CNP was supplemented in the experimental groups and omitted in the control groups. In Experiment 1, CNP was only supplemented at the early stage and follicle development was evaluated. In Experiments 2 and 3, CNP was supplemented during the whole period of in vitro culture. In Experiment 2, follicle development and oocyte maturity were evaluated. In Experiment 3, follicle development and embryo cleavage after in vitro fertilization (IVF) were assessed. The results showed that in the control groups in all three experiments, granulosa cells migrated from within the follicle and the follicles could not reach the antral stage. In the experimental groups in all three experiments, no migration of granulosa cells was observed and follicle development was assessed as attaining the antral stage, which was significantly superior to that of the control group ( P < 0.0001). Oocyte meiotic arrest was effectively maintained, hence giving good developmental competence. In conclusion, CNP supplementation in the culture system during IVG benefited the development of murine preantral follicles.
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