TMOD-16. MULTIFUNCTIONAL NANOPARTICLES USED FOR MAGNETIC RESONANCE/NIR FLUORESCENCE DUAL-MODAL IMAGING AND CHEMOTHERAPY OF GLIOMA

Abstract PURPOSE Glioma is the most common malignant brain tumor and infamous for its high invasiveness and lethality. Therefore, plenty of techniques like intraoperative MRI (iMRI) and fluorescence-guided surgery were performed to improve the surgical resection rate of glioma. In addition, the chemotherapy effect of glioma was severely restricted because of the low blood-brain barrier (BBB) permeability of traditional therapeutic drugs. Doxorubicin (DOX) is a widely used antineoplastic drug, but its application in glioma therapy is prevented by the BBB. Thus, it is urgent to develop novel diagnostic methods and drug delivery systems to improve glioma treatment effect. MATERIALS AND METHODS In this study, we constructed a multifunctional nanotheranostic agent which integrated MR imaging, fluorescence imaging and targeting chemotherapy for glioma by encapsulating iron oxide nanoparticles, DOX and indocyanine green (ICG) with PEGylated phospholipid. RESULTS The obtained multifunctional were proved with an average diameter of 22.9 nm. In vitro cell experiments demonstrated that the nanoparticle effectively enhanced cellular uptake of DOX compared with that of free DOX. In vivo MR and fluorescence imaging not only demonstrated that the nanotheranostic agent effectively crossed the BBB and selectively accumulated at the tumor area, but also easily monitored the real-time therapeutic outcome. Among all treatment groups, C6 glioma-bearing rats treated with nanoparticles exhibited the maximal degree of efficacy, including the longest survival times and smallest tumor volume. CONCLUSION All these results suggested that the multifunctional nanoparticles could be simultaneously used for iMRI and fluorescence-guided surgery to increase the resection rates, as well as delivering chemotheraputic drugs to tumor site to improve therapeutic effect. We believe that this multifunctional nanoparticle might serve as a potential theranostic treatment for glioma in the near future.