菌类
结肠炎
酿酒酵母
肠道菌群
炎症性肠病
尿酸
生物
酵母
微生物学
转录组
免疫学
疾病
医学
生物化学
生态学
内科学
基因表达
基因
作者
Tyson R. Chiaro,Ray Soto,W. Zac Stephens,Jason L. Kubinak,Charisse Petersen,Lasha Gogokhia,Rickesha Bell,Julio Delgado,James E. Cox,Warren P. Voth,Jessica Brown,David J. Stillman,Ryan M. O’Connell,Anne E. Tebo,June L. Round
出处
期刊:Science Translational Medicine
[American Association for the Advancement of Science (AAAS)]
日期:2017-03-08
卷期号:9 (380)
被引量:157
标识
DOI:10.1126/scitranslmed.aaf9044
摘要
The commensal microbiota has an important impact on host health, which is only beginning to be elucidated. Despite the presence of fungal, archaeal, and viral members, most studies have focused solely on the bacterial microbiota. Antibodies against the yeast Saccharomyces cerevisiae are found in some patients with Crohn's disease (CD), suggesting that the mycobiota may contribute to disease severity. We report that S. cerevisiae exacerbated intestinal disease in a mouse model of colitis and increased gut barrier permeability. Transcriptome analysis of colon tissue from germ-free mice inoculated with S. cerevisiae or another fungus, Rhodotorula aurantiaca, revealed that S. cerevisiae colonization affected the intestinal barrier and host metabolism. A fecal metabolomics screen of germ-free animals demonstrated that S. cerevisiae colonization enhanced host purine metabolism, leading to an increase in uric acid production. Treatment with uric acid alone worsened disease and increased gut permeability. Allopurinol, a clinical drug used to reduce uric acid, ameliorated colitis induced by S. cerevisiae in mice. In addition, we found a positive correlation between elevated uric acid and anti-yeast antibodies in human sera. Thus, yeast in the gut may be able to potentiate metabolite production that negatively affects the course of inflammatory bowel disease.
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