Roles of extracellular polymeric substances in the adsorption and removal of norfloxacin during hydrothermal treatment of sewage sludge

Hydrothermal treatment (HT) is promising to remove antimicrobials from sewage sludge (SS); however, the mechanism of antimicrobial degradation during the HT of SS is not fully understood. In this study, the roles of extracellular polymeric substances (EPS) in the removal and transformation of norfloxacin (NOR) during the HT of SS at temperatures of 100 and 160 °C were investigated. The results indicated that the degradation of NOR increased with increasing HT temperature, with maximum NOR removal (52 %) achieved at 160 °C. Furthermore, the NOR in sludge showed higher degradation efficiencies than the control as HT temperature was higher than 120 °C. Evident promotion effects of bound-EPS (B-EPS) in sludge were observed on the NOR degradation as HT temperature was higher than 120 °C, leading to the mineralization and deamination of protein-like components in EPS during HT. Besides, the adsorption capacity of NOR during the HT of SS decreased at temperatures higher than 120 °C. The evolution of the spatial structure of B-EPS was predominantly responsible for the adsorption of antimicrobials, a spontaneous process driven mainly by hydrophilic interactions. With the hydrothermal conversion of B-EPS, the electron transfer, and reactive species (3EPS* and ·OH) derived from B-EPS could facilitate the degradation of NOR. In particular, hydrogen bonds between B-EPS and NOR increased the apparent yield of ·OH and accelerated the decarboxylation of NOR during HT at temperatures higher than 120 °C. A toxicity evaluation suggested that HT for NOR degradation could attenuate toxicity, whereas deep oxidation or mineralization would be needed to promote ecosystem safety. These findings provide new insights into the hydrothermal activation of EPS and the interrelated hydrothermal fate of antimicrobials and other toxic pollutants in sludge.