谷胱甘肽
荧光
赫拉
生物相容性
化学
体内
聚集诱导发射
检出限
生物物理学
细胞内
组合化学
纳米技术
生物化学
体外
材料科学
生物
色谱法
物理
生物技术
有机化学
量子力学
酶
作者
Junxiong Yao,Qian Meng,Han Xu,Chang Xu,Xiaohua Cao,Huanan Huang,Chenglin Bai,Rui Qiao
标识
DOI:10.1016/j.dyepig.2023.111481
摘要
Real-time and accurate detection of intracellular GSH content is crucial for clinical diagnosis, disease treatment, and pathogen detection. Creating fluorescent probes that possess aggregation induced emission (AIE) and precisely detect GSH remains a formidable challenge despite the widespread reporting of numerous fluorescent probes for GSH recognition. This may due to the absence of effective strategies. Herein, using a "turn off - turn on" strategy, a novel fluorescent probe DPND with AIE characteristic has been devised and synthesized. It can detect GSH selectively and uninhibitedly in the presence of various ions. The limit of detection of DPND-Cu2+ for GSH was calculated to be 1.17 μM, demonstrating the probe's ability to detect GSH quantitatively. More crucially, the probe exhibits low toxicity and solid biocompatibility while recognizing GSH in HeLa cells and mices, indicating that it may be a promising candidate for in vivo detection of GSH. Consequently, this work offers the potential to precisely tailor molecular recognition of GSH in addition to providing ongoing exploration of approaches for monitoring GSH.
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