副热带青蟹
溶藻弧菌
生物
蛋白激酶A
白斑综合征
细胞生物学
激酶
先天免疫系统
p38丝裂原活化蛋白激酶
MAP激酶激酶激酶
信号转导
丝裂原活化蛋白激酶
ASK1
丝裂原活化蛋白激酶激酶
微生物学
弧菌
免疫学
生物化学
基因
病毒
免疫系统
细菌
遗传学
作者
Chen-Yang Lin,Yanmei Zhang,Bang-Ze Li,Miao-An Shu,Wenbin Xu
标识
DOI:10.1016/j.dci.2023.104755
摘要
Mitogen-activated protein kinase kinase 4 (MKK4), serves as a critical component of the mitogen-activated protein kinase signaling pathway, facilitating the direct phosphorylation and activation of the c-Jun N-terminal kinase (JNK) and p38 families of MAP kinases in response to environmental stresses. In the current research, we identified two MKK4 subtypes, namely SpMKK4-1 and SpMKK4-2, from Scylla paramamosain, followed by the analysis of their molecular characteristics and tissue distributions. The expression of SpMKK4s was induced upon WSSV and Vibrio alginolyticus challenges, and the bacteria clearance capacity and antimicrobial peptide (AMP) genes’ expression upon bacterial infection were significantly decreased after knocking down SpMKK4s. Additionally, the overexpression of both SpMKK4s remarkably activated NF-κB reporter plasmid in HEK293T cells, suggesting the activation of the NF-κB signaling pathway. These results indicated the participation of SpMKK4s in the innate immunity of crabs, which shed light on a better understanding of the mechanisms through which MKK4s regulate innate immunity.
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