胎牛血清
细胞培养
细胞生长
活力测定
细胞
细胞分化
SH-SY5Y型
细胞生物学
生物
男科
化学
免疫学
分子生物学
医学
神经母细胞瘤
生物化学
遗传学
基因
作者
Zeynep Bengisu Kaya,Valeria Santiago-Padilla,Melina J. Lim,Suelen Lúcio Boschen,Pergin Atilla,Pamela J. McLean
标识
DOI:10.1038/s41598-024-55516-5
摘要
Abstract In the quest to unravel the mysteries of neurological diseases, comprehending the underlying mechanisms is supreme. The SH-SY5Y human neuroblastoma cell line serves as a crucial tool in this endeavor; however, the cells are known for its sensitivity and slow proliferation rates. Typically, this cell line is cultured with 10% Fetal Bovine Serum (FBS) supplement. Nu-Serum (NuS), a low-protein alternative to FBS, is promising to advance cell culture practices. Herein, we evaluated the substitution of NuS for FBS to test the hypothesis that an alternative serum supplement can aid and promote SH-SY5Y cell proliferation and differentiation. Our findings revealed that the NuS-supplemented group exhibited a notable increase in adhered cells compared to both the FBS and serum-free (SF) groups. Importantly, cell viability remained high in both sera treated groups, with the NuS-supplemented cells displaying significantly larger cell sizes compared to the SF-treated group. Furthermore, cell proliferation rates were higher in the NuS-treated group, and neuroblast-like morphology was observed earlier than FBS group. Notably, both FBS and NuS supported the differentiation of these cells into mature neurons. Our data supports NuS as an alternative for SH-SY5Y cell culture, with the potential to elevate the quality of research in the neuroscience field.
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