伤口愈合
角蛋白
纤维连接蛋白
细胞外基质
再生(生物学)
血管生成
角蛋白14
碱性成纤维细胞生长因子
化学
细胞生物学
癌症研究
医学
生长因子
免疫学
病理
生物
生物化学
转基因
转基因小鼠
受体
基因
作者
Changfa Sun,Yuanzhi Huang,Lili Wang,Jia Deng,Rui Qing,Xin Ge,Hongyuan Xue,Guodong Zha,Wei Hua Pu,Bochu Wang,Shilei Hao
标识
DOI:10.1016/j.ijbiomac.2024.129725
摘要
Keratin materials are promising in wound healing acceleration, however, it is a challenge for the keratin to efficiently therapy the impaired wound healing, such as diabetic foot ulcers. Here, we report a keratin/bFGF hydrogel for skin repair of chronic wounds in diabetic rats based on their characteristics of extracellular matrix and growth factor degradation in diabetic ulcer. Recombinant keratin 31 (K31), the most abundant keratin in human hair, exhibited the highly efficient performances in cell adhesion, proliferation and migration. More importantly, the introduction of bFGF into K31 hydrogel significantly enhances the properties of cell proliferation, wound closure acceleration, angiogenesis and skin appendages regeneration. Furthermore, the combination of K31 and bFGF can promote epithelial-mesenchymal transition by inhibiting the expression of E-cadherin and promoting the expression of vimentin and fibronectin. These findings demonstrate the engineered K31/bFGF hydrogel as a promising therapeutic agent for diabetic wound healing.
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