A rhodol-based fluorescent probe for ratiometric sensing of hydrogen sulfide in living cells

Hydrogen sulfide is an important signaling molecule which plays a vital role in many biological processes. In this work, a novel near-infrared FRET (fluorescence resonance energy transfer) fluorescent probe was developed based on the rhodol derivative to detect H2S. The ratio of fluorescent intensity with the concentration of H2S exhibited an excellent linear relationship when probe LT responded to H2S. Probe LT exhibited excellent selectivity, sensitivity and anti-interference. LT was applied to sense endogenous and exogenous H2S in cells with great performance. Moreover, LT had the ability to target endoplasmic reticulum (ER) in Hela cells.