诱导多能干细胞
药品
细胞生物学
人诱导多能干细胞
疾病
生物
干细胞
人类疾病
病理
药理学
医学
胚胎干细胞
遗传学
基因
作者
Lorna J Hale,Melissa H. Little
出处
期刊:Methods in molecular biology
日期:2023-01-01
卷期号:: 55-68
标识
DOI:10.1007/978-1-0716-3179-9_5
摘要
The functional unit of human kidney is the nephronNephrons. This structure is composed of a glomerulus, connected to a tubule that drains into a collecting duct. The cells which make up the glomerulus are critically important to the appropriate function of this specialised structure. Damage to glomerular cells, particularly the podocytes, is the primary cause of numerous kidney diseases. However, access to and the subsequent culture of human glomerular cells is limited. As such, the ability to generate human glomerular cell types from induced pluripotent stem cells (iPSCs) at scale has garnered great interest. Here, we describe a method to isolate, culture and study 3D human glomeruli from induced pluripotent stem cell (iPSC)-derived kidney organoids in vitro. These 3D glomeruli retain appropriate transcriptional profiles and can be generated from any individual. As isolated glomeruli, they have applicability for disease modelling and drug discovery.
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