生物
人口
分子生物学
单克隆抗体
抗体
流式细胞术
巨噬细胞
细胞培养
抗原
肺泡
单元格排序
细胞生物学
免疫学
体外
生物化学
遗传学
社会学
人口学
作者
Kumaresan Sankaran,Herbert B. Herscowitz
摘要
Abstract We have previously reported that MH-S, an established murine alveolar macrophage-derived cell line, mediated profound inhibition of in vitro antibody production, as did their freshly isolated alveolar macro phage (AM) counterparts. In this communication we show that like freshly recovered AMs, the MH-S cell line also displays phenotypic and functional heterogeneity. Sorting of parental MH-S cells by flow cytometry based on reactivity with anti-Mac-1 antibody yielded two sub sets. Further analysis by staining with monoclonal anti bodies against well-characterized murine macrophage cell surface markers revealed that both Mac-1+ and Mac-1-subsets expressed the mature murine macrophage antigen (F4/80) and class II major histocompatibility complex molecules, but with different intensity. In contrast, the two subsets stained equivalently with antibody against the FCγII receptor, whereas neither subset stained with anti-CD4 antibody. Examination by light microscopy revealed pleomorphism in the Mac-1+ population with many of the cells appearing spindle shaped and having elongated processes, whereas a majority of the cells in the Mac-1- population were spherical in shape. Functionally, cells from the Mac-1+ population were less inhibitory of in vitro antibody production and produced significantly more nitric oxide in response to stimulation with lipopolysaccharide than were cells in the Mac-1- population. Essentially similar results were obtained using cloned Mac-1+ and Mac-1- MH-S cells. The finding of het erogeneity in an established cell line that displays functions similar to those of freshly recovered AMs suggests that distinct subsets of AMs may be involved in the pathogenesis of disease processes in the lung. J. Leukoc. Biol. 57: 562–568; 1995.
科研通智能强力驱动
Strongly Powered by AbleSci AI