Operation for Bio-Hydrogen Production of CSTR with Integration of Immobilized and Suspended Cell Process

A new-styled continuous stirred tank reactor (CSTR) seeded with activated sludge attached onto granular activated carbon (GAC) was developed for raised hydrogen production using diluted grenadine syrup. Emphasis was placed on the hydrogen producing performance and stability of the reactor. It was found that the temperature of (35 ± 1) °C, hydraulic retention time (HRT) of 4h, influent COD of 6000mg/L, the maximum hydrogen production was up 12.06L/d. In addition, though pH decreased to 3.42, the microbial growth still high and COD removal rate reached 50%, demonstrating that the reactor processed the ability of acid resistance.